denaturing polyacrylamide gel meaning in Chinese
变性聚丙烯酰胺凝胶
Examples
- The pcr amplification products were analyzed by vertical denaturing polyacrylamide gel electrophoresis followed by silver staining
Ys393二基因座, pag电泳分型,银染显色。 - The pcr amplification products were separated on 6 % vertical denaturing polyacrylamide gels . numerous and distinct bands could be detected by silver staining
Pcr扩增产物经6变性聚丙烯酰胺凝胶垂直电泳分离后,银染能检测到多而清晰的条带。 - Pcr - based analysis method was carried out in the following study . dna amplification by pcr in cgg repeats were performed on 154 people as normal control ( 73 males and 81 females ) and 23 members from four x - linked mental retardation ( xlmr ) families . after electrophoresed on a 6 % - denaturing polyacrylamide gel ( acr : bis = 19 : l ) , genotypes of the objects were determined by analyzing pcr products
本研究采用较以往更为简便有效的pcr技术方法,对随机抽取的154人的正常群体(男性73人、女性sl人)及来自四个智力低下家系的23名成员进行了fmr基因cgg重复序列的扩增,利用变性聚丙烯酸胺凝胶电泳技术对不同等位基因类型进行分离和统计。 - The enzyme was purified from cell extract by ammonium sulfate fractionation ( 30 % - 80 % saturation ) and consecutive column chromatography using deae - cellulose and sephadex g - 100 . the sod activity for purified enzyme could reach 4966 iu / mg proteins , and the molecular weight of the sod was about 20 kda which determined by sds - page electrophoresis . when the non - denaturing polyacrylamide gel stained with substrate for sod in the present of 0 . 2mmol / l kcn or 0 . 5 mmol / l h2o2 , the active band was still showed at the same position , which indicated the sod could not be inhibited by the treatments with kcn or h2o2 and it was the mn sod
通过sds一聚丙烯酸胺凝胶电泳可知该sod酶的分子量约为20kda .在非变性聚丙烯酞胺凝胶( pagb )电泳后,在凝胶son显色反应液中加入0 . 2耐01 / lkcn或0 . 5inmol / lh202 ,凝胶sod显色反应后在原来的sod酶部位仍然含有sod活性带,这表明利用kcn和h20 :处理并不能抑制500的活性,该sod属于mn一sod 。
Related Words
- polyacrylamide
- hydrolyzed polyacrylamide
- polyacrylamide gel
- polyacrylamide resin
- polyacrylamide gel electrophoresis
- polyacrylamide clear agent
- partially hydrolyzed polyacrylamide
- polyacrylamide hydrolysis product
- denaturing gradient polyacrylamide gel
- denature
- denaturing gradient gel electrophoresis
- denaturing gradient polyacrylamide gel
- denaturing protein
- denaturization